Frequently Asked Questions
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You can operate the Concentrator plus as a concentrator, desiccator or as a centrifuge. There are three different modes for various solvents available for the concentrator or desiccator function respectively: for aqueous, for alcoholic solvents and for solvents with high vapor pressure. The concentrator function enables the quick concentration of samples via vacuum (fast evaporation of the solvent) and centrifugation (prevents foaming over/sample loss) in the rotor. The desiccator function enables fast drying under vacuum conditions without using a rotor, e.g. for filter papers. The sample can be placed directly in the rotor chamber; the rotor axle doesn't rotate during the run. With the centrifuge function, the rotor spins, but the rotor chamber is not evacuated. For optimal concentration performance we recommend the settings you find in the chart.
The run time can be set with the time arrow keys. The setting of 1 min to 9:59 hours and endless (∞) is possible.
The temperature steps --, 30, 45, 60 °C can be set using the temp key and are visible on the display, whereby -- symbolizes evacuation without temperature control. The temperature can be changed during operation. The new temperature is immediately readjusted.
The braking function can be set with the brake key. ON is found above this function key on the display when the brake is switched on. The rotor is in this way gently decelerated at the end of the run. Deceleration can also take place without braking. For this purpose push the brake key until OFF appears in the display. It is possible to move between these settings at any time.
The braking function, the temperature and the total run time can be changed. The new parameters are adjusted immediately. During the run, the set function, which can be selected with the mode/vent key, cannot be changed.
The ventilation valve is opened by pressing the mode/vent key during the run. Air flows in and rinses the pump and tube system. Prior to concluding daily work and upon finishing the last run, we recommend pressing the button once for a few seconds in order to remove residual condensation from the pump.
The ambient temperature for the operation of the Concentrator plus complete system may range from 15 °C to a maximum of 35 °C.
The rotational speed for all rotors is 1,400 rpm. It cannot be changed. The g-force varies according to the position of the tubes in the rotors between 130 and 250 x g.
Yes, please contact your local Eppendorf partner. The retrofitting is carried out by Eppendorf Service.
In order to ensure the functionality of the Concentrator plus, an external vacuum pump must have a suction capacity of at least 1.9 m³/h at 50 Hz or 2.1 m³/h at 60 Hz. Each pump used should be able to contain a maximum ultimate pressure of at least <20 hPa (<20 mbar). The vacuum pump should also be resistant to chemicals.
An external vacuum pump with a power input of < 400 W can be connected directly to the basic device with a special plug. A socket is found at the rear of the device. In contrast, an external vacuum pump with a power input of > 400 W must be connected directly to the power supply. The control of the pump by the Concentrator in this case takes place via a solenoid valve located between the vacuum pump and the Concentrator plus.
The inside of the pump is coated with polytetrafluorethylene (PTFE). The feeder from the rotor chamber to the pump is, in the case of the Concentrator complete system, also coated with PTFE .
The lid is of polymethylmethacrylate (PMMA).
Only neutral, gentle cleaning agents and alcohol-based disinfectants may be used for the cleaning and disinfection of the device. This applies for both the housing and the rotor chamber. The mains plug must be disconnected prior to cleaning. It should principally be prevented that aqueous, saline solutions be allowed to dry, and the longer term effects of acids or lyes on the material avoided.
Neutral, gentle cleaning agents and alcohol-based disinfectants may be used for cleaning and disinfection. Where contamination with biologically dangerous elements is suspected, we recommend autoclaving the rotors (121 °C, 20 min).
The small metal feet can be screwed into the corresponding bores of these rotors. They ensure that the tubes are not pushed out of the bores when one places the rotor on the workbench. The rotors can be used in the Concentrator plus with the feet. They should be regularly tightened.
The membrane vacuum pump only reaches the suction capacity and ultimate pressures given in the "Technical Data" after a warm up phase of the Concentrator of approx. 15 min. The warm-up phase reduces the condensation of liquids in the pump and on the lid of the rotor chamber. If condensation should nonetheless accumulate in the pump after large sample throughput, please let the Concentrator run for several minutes following the completion of work.
The time required for the concentration of a sample depends on the properties of the sample (vapor pressure, boiling point, etc.), the quantity of liquid to be concentrated and on the type of vessel. Several examples can be found in the following table.
The Concentrator was developed to concentrate or dry samples with DNA, RNA, nucleotides and proteins, which are found in alcoholic or aqueous solutions.
No. The Eppendorf Concentrator plus may not be used in a potentially explosive environment and must be operated in a safe, adequately ventilated environment or beneath an extraction hood. Chemicals that can potentially create an explosive atmosphere may not be used in the Concentrator plus.
You can connect any commercially available cold trap to our Concentrator plus. The cold trap can be used behind the pump in place of the emission condenser with the supplied tube.
When concentrating flammable, chemically aggressive liquids or those with volatile, chemically aggressive or biologically hazardous components, an effective cold trap or chemical trap must be used in place of the emission condenser. Only this way users can be protected from solvents that are dangerous to health. In the case of the Concentrator complete system, the solvent-resistant pump alows the setup behind the pump.
The PVC tube supplied by Eppendorf, which normally connects the pump and the emission condenser, can be used, for chemicals to which PVC is resistant.
"According to our information, the following cold trap can be recommended: COLD TRAP type KF 29-GL incl. Dewar from the KGW-Isotherm company. An overview of dealers and representatives can be found at their homepage www.kgw-isotherm.de."
Yes, one can centrifuge 0.2 ml tubes in the 1.5 ml rotor F-45-48-11 with the help of an adapter #5425 715.005 (order no North America #022636260), in the 0.5 ml rotor F-45-72-8 with the help of the adapter #5425 723.008 (order no North America #022636286) or in the swing bucket rotor A-2-VC with the help of the work tray for 96 x 0.2 ml PCR tubes (#0030 124.235, order no North America #951010031).
The height of 27 mm for Microtest Plates may not be exceeded.
During centrifugation, the menisci stand angled in the tubes at the edge of the plates. This is caused by the centrifugal force and is unavoidable. Therefore, the wells of the plates should only be filled to a maximum of approx. 2/3 of the max. filling volume.
"When a spacer (#5301 316.005, (order no North America #022822101) is placed between them, two rotors can be centrifuged simultaneously (possible with the rotors F-45-48-11 and F-45-72-8 in desired order, not with rotor F-45-70-11). When using in combination, the maximum load is reduced:
Yes, the Concentrator plus can also be operated purely as a desiccator. The rotor chamber is thereby evacuated normally, but the rotor remains immobile. With the mode/vent key, depending upon the solvent with which the filter plate is treated, the functions D-AQ, D-AL or D-HV can be selected in the display.
On a complete system with junction for external devices, a gel dryer can be run in parallel to the operation as a concentrator/desiccator and/or used separately. For parallel work we recommend the V-HV and D-HV modes. If the gel dryer is run separately, you should use D-HV mode in order to protect the drive. In contrast to the concentrator or desiccator function, open the tap of the gel dryer connection before operation. The knob thereby points in the flow direction. Close it again after operation. When the gel dryer is operated without the concentrator or desiccator function the device lid must also be closed as a vacuum otherwise cannot be generated. Then proceed as with the concentrator function. Please note the solvent properties when carrying out work. When running several devices in parallel on a vacuum system and/or in combination with a gel dryer, an explosive gas mixture can develop in the vacuum system. Only carry out parallel operations with identical solvents or solvents that evaporate in a non-hazardous manner.