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Which format is the right format of cable to connect PC to epMotion?

Can I use each Monitor for epBlue?

Where can I find the current combination of labware for epMotion 5070/75?

What is to do, if my labware is not in the labware list?

How can I install a 2. client?

Do I need an additional epBlue license to install epBlue on a second PC?

How can I install epBlue on a second PC?

What is to do , if the errors Ox0B10 and Ox 1986 appear after the log in?

Log files can only be signed immediately after an application run has ended. They cannot be signed later.

When an application is deleted, the system automatically deletes all ID lists that were created during the execution of this application.

If the user minimizes and restores the epBlue window when the simulator is active, the simulator operating controls can no longer be accessed and the simulator cannot be closed. Then, the user must close the epBlue client using the Task Manager.

If the user has deactivated labware that is used in an application which is currently open, the "Run >>" button may remain active even though the application cannot be executed in this state

If the backup medium (e.g., USB storage medium or network drive) is disconnected during a database backup, epBlue terminates with an unhandled exception.

The length of the labware names is limited to 21 characters. If labware from an earlier version of epBlue A1, A2 or CP is imported and the version includes names with more than 21 characters, the names will be truncated during import.

Can the dispensing tool pipet concentrated HCl?

With which liquid type can I pipet DMSO?

Is it possible to work with ELISA kits on epMotion?

How can I change the preprogrammed liquid types?

Is it possible to upgrade epMotion 5070 Refreher 1 version to Refresher 2 version?

Is it possible to work with a PCR-Cooler on epMotion?

Is it possible to upgrade the epMotion 5070 with a Thermomodule?

Where can I find the current combination of labware for epMotion 5070/75?

Are the Dispensing tools autoclavable?

What is to do, if my labware is not in the labware list?

Can the epMotion automatically pick up plates, transport material and change tools?

How many positions does the epMotion 5070 has?

Can the dispensing tool pipet concentrated HCl?

With which Liquid type can I pipet DMSO?

Is the epMotion 5075 LH upgradable to VAC or MC version?

Is it possible to upgrade epMotion 5075 Refresher 1 version to Refresher 2 version?

Is it possible to work with ELISA kits on epMotion?

How can I change the preprogrammed Liquid types?

Is it possible to work with a PCR-Cooler on epMotion?

Is it possible to upgrade the epMotion 5075 with an additional Thermomodule?

Where can I find the current combination of labware for epMotion 5070/75?

What is to do, if my labware is not in the labware list?

Are the Dispensing tools autoclavable?

Is it possible to use Tubes of other manufacturer for epMotion?

Can the epMotion automatically pick up plates, transport material and change tools?

What purpose does the optical sensor serve?

When measuring the concentration of sample DNA from the MagSep Viral DNA/RNA kit by UV-Vis spectroscopy I always see the same concentration OD (260 nm) for all samples?

Is a ratio OD (260 nm)/ (280 nm) between 2.0 and 2.2 good?

I see different yield from my blood samples when I compare the MagSep kit with competitive kit methods?

In the down stream application no EDTA is wanted in the eluted gDNA. What can be done?

How often can tips be re-used with the tips function of epMotion?

Will the HEPA and UV be something that can added later?

How often can tips be re-used with the tips function of epMotion?

What source is the carrier RNA in the MagSep viral DNA/RNA kit from?

Can the MagSep blood gDNA kit be applied with heparine treated blood samples?

What do I have to do if my blood samples do not have 200 µl volume?

Can one of the MagSep kits (tissue and cells, blood or viral) be used to purify nucleic acid from buccal swabs?

Is the gDNA tissue and cells kit compatible with bacterial cell extractions?

What is included in the P5073 product shipment?

Is it possible to integrate thermomodules into P5073?

Is it possible to integrate thermomodules into M5073?

Do we also offer TMX retrofit for the P5073? Can we have both thermalmodule and TMX on P5073?

What is the origin of Proteinase K delivered with the kit?

Are the MagSep kits shipped on dry ice?

What is to do, if new loaded labware is not to see in the labware folders?

What is to do, if new loaded labware is not to see in the labware folders?

What is to do, if new programmed application (under epBlue Studio) could not save?

What is to do, if new programmed application (under epBlue Studio) could not save?

Are batch-specific certificates for the new purity grades available in the internet?

Why are there double labels on the folding boxes, as "PCR clean" + "Sterile" or "PCR clean" + "Protein-free"?

Are Eppendorf products free of RNA?

Why are there new Eppendorf purity grades in 2012?

Have the products changed with the new purity grades?

Which purity grades will be modified from 2012?

Can products of the previous and new modular purity grades (with new seals) be used simultaneously in one application?

Which format is the right format of cable to connect PC to epMotion?Back to top
The cable is an ethernet cable RJ 45 Crooslink cable
Can I use each Monitor for epBlue?Back to top
Eppendorf recommend a 19" Monitor.
Where can I find the current combination of labware for epMotion 5070/75?Back to top
You can find it under www.epmotion.com.
What is to do, if my labware is not in the labware list?Back to top
Contact your local epMotion Specialist..
How can I install a 2. client?Back to top
Please read the Userguide 016.
Do I need an additional epBlue license to install epBlue on a second PC?Back to top
No, there is no additional license needed.
How can I install epBlue on a second PC?Back to top
The epBlue package includes a CD to install epBlue on a second PC.
What is to do , if the errors Ox0B10 and Ox 1986 appear after the log in?Back to top
In the name of new labware or new labware combinations are forbidden signs like §,& or others. Please, contact your local epMotion Specialist to repair that.
Log files can only be signed immediately after an application run has ended. They cannot be signed later.Back to top
Do not close the "Signature" dialog for the log file until the last required signature has been completed.
When an application is deleted, the system automatically deletes all ID lists that were created during the execution of this application.Back to top
Export ID lists in advance as files
If the user minimizes and restores the epBlue window when the simulator is active, the simulator operating controls can no longer be accessed and the simulator cannot be closed. Then, the user must close the epBlue client using the Task Manager.Back to top
Do not minimize the epBlue window when the simulator is active
If the user has deactivated labware that is used in an application which is currently open, the "Run >>" button may remain active even though the application cannot be executed in this stateBack to top
Close the application before deactivating the labware.
If the backup medium (e.g., USB storage medium or network drive) is disconnected during a database backup, epBlue terminates with an unhandled exception.Back to top
Do not remove the backup medium during the backup.
The length of the labware names is limited to 21 characters. If labware from an earlier version of epBlue A1, A2 or CP is imported and the version includes names with more than 21 characters, the names will be truncated during import.Back to top
The importer outputs a corresponding text notification so the user can modify his applications.
Can the dispensing tool pipet concentrated HCl?Back to top
Yes.
With which liquid type can I pipet DMSO?Back to top
DMSO can be pipet with the Liquid type "Alcohol 98%", see Application note 155.
Is it possible to work with ELISA kits on epMotion?Back to top
Yes, see Application note 138.
How can I change the preprogrammed liquid types?Back to top
In the parameters of a command you can find the point "Options". Under this point is the"Liquid type". Under "Liquid type" you can click on the point "Change parameter".Then you can change the preprogrammed values till you find the optical parameters for the particular liquids.
Is it possible to upgrade epMotion 5070 Refreher 1 version to Refresher 2 version?Back to top
No, it isn't possible. But you have the possibility to replace the Refresher 1 device and get back a Refresher 2 version 5070 at extra charge.
Is it possible to work with a PCR-Cooler on epMotion?Back to top
Yes, it's possible, if you work with the Thermoadapter "Frosty" (Order no.5075 789.000).
Is it possible to upgrade the epMotion 5070 with a Thermomodule?Back to top
No, you can integrate Thermomodules in epMotion 5075, only.
Where can I find the current combination of labware for epMotion 5070/75?Back to top
You can find it under www.epmotion.com.
Are the Dispensing tools autoclavable?Back to top
Yes.
What is to do, if my labware is not in the labware list?Back to top
Contact your local epMotion Specialist.
Can the epMotion automatically pick up plates, transport material and change tools?Back to top
Yes, the epMotion 5075 can transport plates and other things, also it can change the tools automatically. The epMotion 5070 is a semi-automatically system and not able to transport labware.
How many positions does the epMotion 5070 has?Back to top
The working area contains 4 positions. Also, the epMotion 5070 has 3 virtual places outside of the device (Park positions).
Can the dispensing tool pipet concentrated HCl?Back to top
Yes
With which Liquid type can I pipet DMSO?Back to top
DMSO can be pipet with the Liquid type "Alcohol 98%", see Application note 155.
Is the epMotion 5075 LH upgradable to VAC or MC version?Back to top
Yes, it is. The LH version is upgradable to VAC or MC version anytime.
Is it possible to upgrade epMotion 5075 Refresher 1 version to Refresher 2 version?Back to top
Yes, it's possible. Therefore you need the Software and Hardware Upgrade Set 1, Order no. 5075 851.440.
Is it possible to work with ELISA kits on epMotion?Back to top
Yes, see Application note 138.
How can I change the preprogrammed Liquid types?Back to top
In the parameters of a command you can find the point "Options". Under this point is the"Liquid type". Under "Liquid type" you can click on the point "Change parameter".Then you can change the preprogrammed values till you find the optical parameters for the particular liquids.
Is it possible to work with a PCR-Cooler on epMotion?Back to top
Yes, it's possible, if you work with the Thermoadapter"Frosty" Order no.5075 789.000
Is it possible to upgrade the epMotion 5075 with an additional Thermomodule?Back to top
Yes, you can integrate up to 3 Thermomodules in epMotion 5075.
Where can I find the current combination of labware for epMotion 5070/75?Back to top
You can find it under www.epmotion.com.
What is to do, if my labware is not in the labware list?Back to top
Contact your local epMotion Specialist..
Are the Dispensing tools autoclavable?Back to top
Yes.
Is it possible to use Tubes of other manufacturer for epMotion?Back to top
Yes, it's possible, to work with different epMotion racks in different sizes. Also it's possible to work with rack from other manufacturer, if they are in SBS format.
Can the epMotion automatically pick up plates, transport material and change tools?Back to top
Yes, the epMotion 5075 can transport plates and other things, also it can change the tools automatically.. The epMotion 5070 is a semi-automatically system and not able to transport labware.
What purpose does the optical sensor serve?Back to top
The optical sensor has three different functions. 1. To scan number of tips and kind of boxes. 2. Labware recognition on the worktable. 3. Amount of liquid in different tubes.
When measuring the concentration of sample DNA from the MagSep Viral DNA/RNA kit by UV-Vis spectroscopy I always see the same concentration OD (260 nm) for all samples?Back to top
The viral LB (lysis Buffer) contains significant amount of Carrier RNA, which will be measured by UV-Vis analysis. A better way of quantification for viral RNA/DNA is real-time PCR.
Is a ratio OD (260 nm)/ (280 nm) between 2.0 and 2.2 good?Back to top
Such ratio between 2.0 and 2.2 can be an indication for RNA contamination of the gDNA.
I see different yield from my blood samples when I compare the MagSep kit with competitive kit methods?Back to top
When comparing different kits and methods it is important to measure the same samples at the same time. White blood cell content varies significantly from sample to sample and from day to day.
In the down stream application no EDTA is wanted in the eluted gDNA. What can be done?Back to top
Replace the elution buffer of the MagSep reagent kit with destilled water.
How often can tips be re-used with the tips function of epMotion?Back to top
The tips can be maximal 6 times be re-used.
Will the HEPA and UV be something that can added later?Back to top
CleanCap, UV-HEPA can not added later. The option need to be ordered upfront and is then manufectured with the specific system.
How often can tips be re-used with the tips function of epMotion?Back to top
The tips can be maximal 6 times be re-used.
What source is the carrier RNA in the MagSep viral DNA/RNA kit from?Back to top
The carrier RNA is synthetic poly(-A) RNA: (poly(A) potassium salt, prepared from ADP with polynucleotide phosphorylase).
Can the MagSep blood gDNA kit be applied with heparine treated blood samples?Back to top
Yes, but after the purification some residual heparine might be present in the gDNA eluat. To minimze heparine influence in downstream assays we recommend to dilute the eluate by 1 to 10 with PCR grade water.
What do I have to do if my blood samples do not have 200 µl volume?Back to top
Within a +/- 10 % volume tolerance of the blood sample is ok for usage with the MagSep blood gDNA kit. If the volume is below 180 µl we recommend to fill up by PBS buffer to 200 µl.
Can one of the MagSep kits (tissue and cells, blood or viral) be used to purify nucleic acid from buccal swabs?Back to top
Yes, you can do that with the MagSep tissue kits.
Is the gDNA tissue and cells kit compatible with bacterial cell extractions?Back to top
Yes, MagSep tissue gDNA is compatible with bacterial DNA (but not directly for plasmid prep).
What is included in the P5073 product shipment?Back to top
1 TS_50, 1 TS_300, waste tub, Thermoblock 96, rack for 0,5/1,5 and 2 ml tubes and 25 adapter for 0,5 ml reaction tubes.
Is it possible to integrate thermomodules into P5073?Back to top
Yes, it is possible to integrate one thermomodule.
Is it possible to integrate thermomodules into M5073?Back to top
No it is not possible to integrate a thermomodule, because it is not enough space.
Do we also offer TMX retrofit for the P5073? Can we have both thermalmodule and TMX on P5073?Back to top
No, it is not possible to have both modules in one system.
What is the origin of Proteinase K delivered with the kit?Back to top
Proteinase K is biological material purified from the origin Tritirachium album. It is not genetically modified.
Are the MagSep kits shipped on dry ice?Back to top
No, they are shipped and stored at Roomtemperature.
What is to do, if new loaded labware is not to see in the labware folders?Back to top
Shut down and switch on the device, again and the new labware datafiles are visible.
What is to do, if new loaded labware is not to see in the labware folders?Back to top
Shut down and switch on the device, again and the new labware datafiles are visible.
What is to do, if new programmed application (under epBlue Studio) could not save?Back to top
Create a folder first and then create an application, otherwise the new application could not save.
What is to do, if new programmed application (under epBlue Studio) could not save?Back to top
Create a folder first and then create an application, otherwise the new application could not save.
Are batch-specific certificates for the new purity grades available in the internet?Back to top
Yes, the batch-specific purity grades can be downloaded at www.eppendorf.com/certificates.
Why are there double labels on the folding boxes, as "PCR clean" + "Sterile" or "PCR clean" + "Protein-free"?Back to top
Due to the modularization of purity grades, some products are labeled with more than one purity grade symbol. For example, the epDualfilter T.I.P.S. has the double seal "PCR clean "+ "Sterile" and the UVette the double seal "PCR clean" + "Protein-free" on the packing. The certification remains unaffected by the modularization.
Are Eppendorf products free of RNA?Back to top
All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.
Why are there new Eppendorf purity grades in 2012?Back to top
The previous purity grades were developed due to customer requirements and new applications, in the course of the years. To meet the customer requirements for single applications even better, Eppendorf has extended the certification of the purity grades "Sterile" (now, also certified as free of pyrogenes) and "Biopur" (now, also certified as free of DNase and PCR inhibitors). In addition, the previous purity grades have been transferred to a new modular system that provides the same variety and flexibility and yet is readily understandable.
Have the products changed with the new purity grades?Back to top
No, they are the same products as before, i.e. all specifications as dimensions remain the same. Only the packing has changed, due to the new seals. In addition, the certification of "Sterile" and "Biopur" has been extended but the entire production process was maintained.
Which purity grades will be modified from 2012?Back to top
For all purity grades, there will be new packing seals. In addition, there will be a further certification for the purity grades "Sterile" and "Biopur". "Sterile" will certify sterility and also the freedom of pyrogenes. The purity grade "Biopur" also includes the freedom of DNase as well as of PCR inhibitors.
Can products of the previous and new modular purity grades (with new seals) be used simultaneously in one application?Back to top
Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.
 
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