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Are batch-specific certificates for the new purity grades available in the internet?

Why are there double labels on the folding boxes, as "PCR clean" + "Sterile" or "PCR clean" + "Protein-free"?

Why are there new Eppendorf purity grades in 2012?

Have the products changed with the new purity grades?

Which purity grades will be modified from 2012?

Can products of the previous and new modular purity grades (with new seals) be used simultaneously in one application?

Can Mastertips be sterilized?

Do Mastertips contain silicone oil?

Are Mastertips resistant to triethylamine?

Which Eppendorf pipettes can be used to pipette GELoader Tips?

What is the diameter of the tip of GELoader?

What is the maximum volume that can be pipetted with a GELoader Tip?

Can the 50 ml adapter for Combitips Biopur be used more than once if it is cleaned after usage with sodium hypochlorite and then with sterile water?

Does the blister packaging of the Biopur products contain latex?

Are the epTIPS Biopur suitable for safe use in the food industry within Europe?

Are the epTIPS Biopur suitable for safe use in the food industry in the USA?

Are Eppendorf products free of RNA?

How do you make your Biopur products free of pyrogens?

How do you make your Biopur products free of pyrogens?

How do you make your Biopur products free of pyrogens?

What are the reasons for using single-use plasticware in the laboratory?

How does one recognize original Eppendorf tips?

Is there a certificate for epTIPS?

How long is the shelf-life of epTIPS?

Can I store Eppendorf pipette tips in a warehouse with temperatures as low as -10°C in winter for a long period?

What are the dimensions of the epTIPS standard?

How are Eppendorf pipette tips tinted?

Is a reload available for the 5 ml epTIPS Box?

Are the polypropylene pipette tips UV-resistant?

If reloads of new epTIPS are to be autoclaved, does the film packaging have to be removed for the purpose?

Are epTIPS electrically neutral?

Are Eppendorf tips antistatic?

Does Eppendorf offer silicone-coated tips?

Does Eppendorf supply heparinized tips?

Are the tips free of latex?

Are Eppendorf pipette tips free of silicone / silicone oil?

To which temperature can solutions be dispensed with epTIPS?

Can a liquid with a viscosity of 1.5 mPas*s be pipetted with Eppendorf pipette tips?

Are the tips suitable for pipetting 80 °C warm mercury.

Are nose cone filters available for Eppendorf pipettes?

How often can the epTIPS Boxes be autoclaved?

Must the lid on epTIPS Boxes be opened for autoclaving?

What may lead to epTIPS boxes becoming deformed during autoclaving?

Can the epTIPS Boxes be autoclaved at 133 °C?

Can the epTIPS Boxes be autoclaved at 133 °C?

To destroy prions, an autoclave step at 136 °C and 3 atm must be carried out. Can the epTIPS Box (or epTIPS Rack Biopur) withstand this?

Is the epTIPS Box or are the epTIPS Racks UV-resistant?

Are 1.5 ml Eppendorf Safe-Lock Tubes safe for use in the food industry in Europe?

What significance does the red dot on the carton for Eppendorf Biopur® products have?

How much serum evaporates from a Eppendorf Safe-Lock Tube at -20 to -70 °C?

Are the non-amber colored Eppendorf Safe-Lock Tubes UV resistant?

What is the life expectancy of Eppendorf micro test tubes?

From what material are your micro test tubes manufactured?

Do you offer siliconized micro test tubes?

Can Eppendorf micro test tubes be sterilized with ethylene oxide?

Can your micro test tubes be frozen at -80 °C?

Can your micro test tubes be coated with acrylic resin within the framework of a UV polymerization?

Can contamination of micro test tubes by bacterial endotoxin be inactivated by autoclaving?

Which Eppendorf micro test tubes are free of DNA?

What level of protein binding affinity do your micro test tubes demonstrate?

How do you define the temperature entry "RT", for example, as a storage condition for your micro test tubes?

What is the best way to label Eppendorf micro test tubes?

Do your micro test tubes contain heavy metals?

Are 1.5 ml Eppendorf Safe-Lock Tubes safe for use in the food industry in USA?

Do Eppendorf Safe-Lock Tubes contain polyethylene glycol (PEG)?

In which temperature range can the Eppendorf Safe-Lock Tubes be used?

To what extent are the amber colored Eppendorf Safe-Lock Tubes permeable to fluorescent dyes?

Can Eppendorf micro test tubes be stored in liquid nitrogen?

Can the Micro Test Tubes 3810X be stored for 3 months at 90 °C?

Can the lid of the Standard Micro Test Tube 3810X be pierced as easily as that of the Eppendorf Safe-Lock Tube?

From what material is the Micropestle manufactured?

Can the Micro Rack 3821 be stored at -86 °C?

From what material is the Rack 3821 made?

What material is the Tube Rack made of?

Is it possible that nitrogen from the tube material penetrates my sample?

Do the Eppendorf PCR consumables have a special grade of purity?

Can the deep well mats be autoclaved?

I would like to seal my deep well plate with a heat sealing foil. Can I also puncture the foil with a multi-canal pipette?

Which of the Eppendorf PCR 96 Plates can be sealed with the heat sealer?

What is the best sealing procedure for storing plates at - 80 °C?

What is the best way to seal a PCR plate?

What are Maxitips for Varipette 4720 made of?

What are the dimensions of the Maxitips S and G?

Are the Eppendorf LoBind Tubes autoclavable?

What distinguishes the DNA LoBind Tube from a "Standard" Eppendorf Safe-Lock Tube?

Do the Eppendorf LoBind Tubes have the same material durability as all other micro test tubes from Eppendorf?

Are the Eppendorf LoBind Tubes coated?

Can I use the Protein LoBind Tubes regardless of whether I am working with basic or acidic proteins?

Are Eppendorf Deepwell Plate autoclavable?

Are Eppendorf Deepwell Plate available with Barcode?

Eppendorf Deepwell Plate are withstand high centrifugation forces – what are the limits?

Are Eppendorf Deepwell Plate LoBind coated or siliconized?

Does the polypropylene of the plates contain plasticizers?

What are the precise dimensions of Eppendorf Deepwell Plate?

Are Eppendorf Deepwell Plate together with the colored border resistant against organic solvents?

How are the sterile Eppendorf Deepwell Plate packed?

Is it possible to store Eppendorf Deepwell Plate at –196°C in liquid nitrogen?

Are the ep T.I.P.S. LoRetention autoclavable?

Under which conditions will the use of ep T.I.P.S. LoRetention be advantageous?

Are the ep T.I.P.S. LoRetention siliconized or otherwise coated?

Are the ep T.I.P.S. LoRetention resistant to organic solvents?

How are the boxes containing ep T.I.P.S. LoRetention distinguishable from those containing other ep T.I.P.S.?

Will foam formation, which is observed during pipetting of detergent containing solutions, be reduced when epT.I.PS. LoRetention are used?

Which advantages do Eppendorf Polypropylene Microplates have over plates made from polystyrene?

Are Eppendorf PP Microplates resistant to organic solvents, e.g. DMSO?

Are the colored rim and labeling resistant to organic solvents?

How may the Eppendorf PP Microplates be sealed?

Which advantages do the black Eppendorf PP Microplates offer?

Which advantages do the white Eppendorf PP Microplates offer?

May the Eppendorf PP Microplates be stored at –196°C in liquid nitrogen?

May the Eppendorf PP Microplates be autoclaved?

Are the Eppendorf PP Microplates in LoBind quality coated or siliconized?

How high may Eppendorf PP Microplates be centrifuged?

May immunoassays such as ELISA be performed in the Eppendorf PP Microplates?

Is it possible to perform absorption measurements in the Eppendorf PP Microplates?

Why are the Eppendorf PP Microplates offered in Sterile and PCR clean, but not in Eppendorf quality?

Are Eppendorf PP Microplates also available with barcode?

What are Combitips made of?

How are the Combitips tested during production?

What purity grades are available for the Combitips advanced?

Which certificates are available for Combitips?

Are Standard Combitips also sterile?

Are Combitips autoclavable?

Are the Biopur Combitips tested according to USP and EP?

Which adapter is required for the 25 ml Combitip?

Which adapter is required for the 50 ml Combitip?

Can the adapter for the 25 ml and 50 ml Combitip be cleaned using sodium hypochlorite?

Does a Combitip always have to be filled to the total volume?

Can Combitips be used in food analysis?

Are Combitips resistant to nonane and dodecane?

Are Combitips resistant to acetonitrile?

Are Combitips resistant to acids?

Are Combitips resistant to trifluoroacetic acid?

Are Combitips resistant to PEG 400 (polyethylene glycol)?

Are the Combitips resistant against petroleum spirit N28?

Can I aspirate liquid from a 1 liter bottle with a 50 ml Combitip without filling the liquid into a beaker first?

Can the Combilong be autoclaved?

What are Combitip Racks made of?

Are Combitip Racks autoclavable?

When must the Combitips advanced adapter for 25 ml and 50 ml have to be changed?

Are Combitips advanced free from slip agents?

Is there a certificat available regarding trace metalls?

Is there a certificat available regarding slip agents (including oleamide, erucamide, stearamide),biocides (including DiHEMDA), plasticizers (phthalates),BSE/TSE free?

What types of applications can be carried out with Eppendorf Tubes® 5.0 mL?

Is it necessary to buy new equipment to be able to use the new tube?

What are the advantages of using Eppendorf Tubes® 5.0 mL compared with conical 15 mL screw-cap tubes?

Is it possible to store samples in 5.0 mL Eppendorf Tubes® in the freezer?

How resistant are the Eppendorf Tubes® 5.0 mL to chemicals?

Are the Eppendorf Tubes® 5.0 mL available with the same purities and LoBind variants as the small micro centrifugation tubes from Eppendorf?

Can the universal adapter for conical 15 mL screw-cap tubes be used in each rotor/adapter that is designed for this tube type?

What is the Tube Clip used for?

Up to which g-force can the Eppendorf Tubes® 5.0 mL be centrifuged?

Which cleaning and disinfection agents can be recommended for cleaning and disinfection of ThermoMixer C, FP, F1.5 and ThermoStat C?

Is the housing of ThermoMixer C, FP, F1.5 and ThermoStat C stable against UV irradiation?

Which DNA and RNAse deconatmination agents can be recommended for decontamination of the devices and the SmartBlocks?

Which decontamination agent do you recommend to remove radioactivity from the devices and SmartBlocks?

Are start and end position of the SmartBlocks the same?

How can the temperature accuracy be verified?

Can cell culture plates be used in the SmartBlock plates?

Can ThermoMixer C, F1.5, FP & ThermoStat C be operated with remote controle?

Can ThermoMixer C, F1.5, FP & ThermoStat C be used in a cold room?

Is it possible to put the SmartBlocks for a rapid cool down into a -20°C freezer?

ThermoMixer C; ThermoStat C: Is it possible to run more than one program in automatic succession?

Which SmartBlocks and vessels can be used with ThermoMixer F1.5 and FP?

The ThermoMixer / ThermoStat shows the error message “ThermoTop not recognized”.

Can PCR plates be used in SmartBlock plates respectively ThermoMixer FP?

Can the ThermoStat C be used in a cold room?

The ep T.I.P.S. Dualfilter have the highest efficiency in the market. Has this changed with the ep T.I.P.S. SealMax?

If I did a mistake the sample touched the filter and the filter blocks. Do I have to throw away the sample together with the tip?

Has the position of the filter changed if compared to ep Dualfilter T.I.P.S. ?

Is there any difference of aerosol penetration rate in comparison to ep Dualfilter T.I.P.S?

Does the self-sealing mechanism of the filter work with all kinds of liquids?

May I use the ep Dualfilter T.I.P.S. SealMax for several pipetting processes, also if the filter came into contact with the liquid?

Is there a time limit for the recovery of sample liquid when accidentally over-pipetting happened?

Are batch-specific certificates for the new purity grades available in the internet?Back to top
Yes, the batch-specific purity grades can be downloaded at www.eppendorf.com/certificates.
Why are there double labels on the folding boxes, as "PCR clean" + "Sterile" or "PCR clean" + "Protein-free"?Back to top
Due to the modularization of purity grades, some products are labeled with more than one purity grade symbol. For example, the epDualfilter T.I.P.S. has the double seal "PCR clean "+ "Sterile" and the UVette the double seal "PCR clean" + "Protein-free" on the packing. The certification remains unaffected by the modularization.
Why are there new Eppendorf purity grades in 2012?Back to top
The previous purity grades were developed due to customer requirements and new applications, in the course of the years. To meet the customer requirements for single applications even better, Eppendorf has extended the certification of the purity grades "Sterile" (now, also certified as free of pyrogenes) and "Biopur" (now, also certified as free of DNase and PCR inhibitors). In addition, the previous purity grades have been transferred to a new modular system that provides the same variety and flexibility and yet is readily understandable.
Have the products changed with the new purity grades?Back to top
No, they are the same products as before, i.e. all specifications as dimensions remain the same. Only the packing has changed, due to the new seals. In addition, the certification of "Sterile" and "Biopur" has been extended but the entire production process was maintained.
Which purity grades will be modified from 2012?Back to top
For all purity grades, there will be new packing seals. In addition, there will be a further certification for the purity grades "Sterile" and "Biopur". "Sterile" will certify sterility and also the freedom of pyrogenes. The purity grade "Biopur" also includes the freedom of DNase as well as of PCR inhibitors.
Can products of the previous and new modular purity grades (with new seals) be used simultaneously in one application?Back to top
Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.
Can Mastertips be sterilized?Back to top
Yes, they can be autoclaved once at 121 °C, 1 bar.
Do Mastertips contain silicone oil?Back to top
No. No silicone is used in the production of Mastertips. The plastics used likewise contain no silicone.
Are Mastertips resistant to triethylamine?Back to top
Yes.
Which Eppendorf pipettes can be used to pipette GELoader Tips?Back to top
Because the maximum volume of the GELoader Tips amounts to 20 µl, pipettes with a grey button can be used, e.g. Reference 2-20 µl.
What is the diameter of the tip of GELoader?Back to top
The outer diameter is 0.3 mm and the inner diameter is approx. 0.12 mm.
What is the maximum volume that can be pipetted with a GELoader Tip?Back to top
The maximum volume of the GELoader Tips is 20 µl.
Can the 50 ml adapter for Combitips Biopur be used more than once if it is cleaned after usage with sodium hypochlorite and then with sterile water?Back to top
The adapter is made of polypropylene (PP). PP is resistant to sodium hypochlorite at room temperature over very lengthy periods.
Does the blister packaging of the Biopur products contain latex?Back to top
No, the blister packaging is free from latex.
Are the epTIPS Biopur suitable for safe use in the food industry within Europe?Back to top
The epTIPS Biopur are suitable for use in the food industry according to the Food and Consumer Goods Legislation § 5, paragraph 1 no. 1 (food and consumer goods). Please note: The suitability of a consumer item for the intended purpose, the influence of the filler on taste and smell, and the compliance with limitations (e.g. global migration, specific limitations and other analytical specifications) should be tested by the manufacturer or user for individual cases.
Are the epTIPS Biopur suitable for safe use in the food industry in the USA?Back to top
The composition of the epTIPS Biopur fulfills the requirements of the FDA regulation 21 CFR § 177,1520 "Olefin polymers". Please note: The suitability of a consumer item for the intended purpose, the influence of the filler on taste and smell, and the compliance with limitations (e.g. global migration, specific limitations and other analytical specifications) should be tested by the manufacturer or user for individual cases.
Are Eppendorf products free of RNA?Back to top
All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.
How do you make your Biopur products free of pyrogens?Back to top
All Biopur products are manufactured fully automatically under cleanroom conditions. This eliminates contamination of the disposables through contact with people. Beta radiation is used as the sterilization method. An independent laboratory checks that each individual batch is free of pyrogens.
How do you make your Biopur products free of pyrogens?Back to top
All Biopur products are manufactured fully automatically under cleanroom conditions. This eliminates contamination of the disposables through contact with people. Additionally the products are sterilized by radiation or treatment with ethylene oxide. An independent laboratory checks that each individual batch is free of pyrogens.
How do you make your Biopur products free of pyrogens?Back to top
All Biopur products are manufactured fully automatically under cleanroom conditions. This eliminates contamination of the disposables through contact with people. Beta radiation is used as the sterilization method. An independent laboratory checks that each individual batch is free of pyrogens.
What are the reasons for using single-use plasticware in the laboratory?Back to top
Contamination and cross-over can only be avoided by the use of single-use plastic items. In addition there is no annoying and time-consuming cleaning of glassware.
How does one recognize original Eppendorf tips?Back to top
All Eppendorf tips have "Eppendorf" and nest form markings on the work cone.
Is there a certificate for epTIPS?Back to top
Yes, a general quality certificate is available for epTIPS, which is included in the delivery package.
How long is the shelf-life of epTIPS?Back to top
We guarantee for a shelf-life of 5 years.
Can I store Eppendorf pipette tips in a warehouse with temperatures as low as -10°C in winter for a long period?Back to top
Like all our single-use plastic items, our pipette tips are also made of polypropylene and can therefore be stored at -10°C even for many years. Please note that you should keep the pipette tips for several minutes at room temperature before pipetting.
What are the dimensions of the epTIPS standard?Back to top
0,1-10 µl: length: 34 mm, diameter work cone: 6 mm, diameter opening: 0,35 mm
0,1-20 µl: length: 40,5 mm, diameter work cone: 6 mm, diameter opening: 0,36 mm
0,5-20 µl: length: 45,7 mm, diameter work cone: 6 mm, diameter opening: 0,37 mm
2-200 µl: length: 52,7 mm, diameter work cone: 7 mm, diameter Öffnung: 0,52 mm
20-300 µl: length: 55 mm, diameter work cone: 7 mm, diameter opening: 0,6 mm
100-1000 µl: length: 70,72 mm, diameter work cone: 8,8 mm, diameter opening: 0,83 mm
1250 µl: length: 75,5 mm, diameter work cone: 8,8 mm, diameter opening: 1,0 mm
2,5 ml: length: 115 mm, diameter work cone: 12 mm, diameter opening: 1,32 mm
5 ml: length: 120,3 mm, diameter work cone: 15,5 mm, diameter opening: 1,65 mm
How are Eppendorf pipette tips tinted?Back to top
Organic, heavy-metal-free dyes (PP granulate) are used to tint the pipette tips.
Is a reload available for the 5 ml epTIPS Box?Back to top
No, the 5 ml epTIPS Box will be filled with epTIPS Standard.
Are the polypropylene pipette tips UV-resistant?Back to top
Yes. Use on a sterile bench, for example, is therefore completely safe.
If reloads of new epTIPS are to be autoclaved, does the film packaging have to be removed for the purpose?Back to top
Yes, under no circumstances should the film be autoclaved as well.
Are epTIPS electrically neutral?Back to top
The tips are electrically neutral. There are no charged particles on the surface. It is therefore unlikely that charged particles like ions or ionic compounds will get caught on the walls.
Are Eppendorf tips antistatic?Back to top
We use absolutely static-free polypropylene, with the result that Eppendorf tips have a surface with maximum hydrophobia and thus ideal draining properties.
Does Eppendorf offer silicone-coated tips?Back to top
No.
Does Eppendorf supply heparinized tips?Back to top
No. We only supply heparinized end-to-end capillaries for glucose determination using EBIO.
Are the tips free of latex?Back to top
Yes, this is clear from the details about substances contained in the safety/technical data sheets.
Are Eppendorf pipette tips free of silicone / silicone oil?Back to top
Yes.
To which temperature can solutions be dispensed with epTIPS?Back to top
The epTIPS of PP are suitable for liquids with a temperature of max. 90°C.
Can a liquid with a viscosity of 1.5 mPas*s be pipetted with Eppendorf pipette tips?Back to top
We are not able to make any generally applicable statements. In addition to the viscosity the surface tension also influences the pipettability of a liquid. In the case of doubt, the customer should determine the error for pipetting the liquid in question by weighing (with known density).
Are the tips suitable for pipetting 80 °C warm mercury.Back to top
No, we advise against this application.
Are nose cone filters available for Eppendorf pipettes?Back to top
Nose cone filters are filters which are pushed into the nose cone. These are available for the Research 10 ml pipette.
How often can the epTIPS Boxes be autoclaved?Back to top
The box can be autoclaved up to 100 times, the reloads up to 5 times.
Must the lid on epTIPS Boxes be opened for autoclaving?Back to top
No, the boxes can be autoclaved with the lid closed.
What may lead to epTIPS boxes becoming deformed during autoclaving?Back to top
The geometry of the epBox housing changes when the box(es) is (are) forced into the autoclave and the door of the autoclave can only be closed with a degree of force. Once the autoclave has cooled down, the deformed shape is retained. Stacking of many boxes should also be avoided. What is more, autoclaving temperature should be checked regularly. Experience suggests that autoclaves are frequently operated at excessive temperatures. The permissible temperature quoted by Eppendorf should never be exceeded.
Can the epTIPS Boxes be autoclaved at 133 °C?Back to top
No, the epTIPS Box cannot be autoclaved at 133 °C. We recommend 121 °C.
Can the epTIPS Boxes be autoclaved at 133 °C?Back to top
No, the epTIPS Box cannot be autoclaved at 133 °C. We recommend 121 °C.
To destroy prions, an autoclave step at 136 °C and 3 atm must be carried out. Can the epTIPS Box (or epTIPS Rack Biopur) withstand this?Back to top
No, the epTIPS Box or the epTIPS Rack Biopur cannot be autoclaved at 136 °C.
Is the epTIPS Box or are the epTIPS Racks UV-resistant?Back to top
The UV stability of the existing epTIPS Box of polycarbonate and of the existing epTIPS Racks of polypropylene were tested during development. These were thereby irradiated with a lamp of 254 nm and energy of approx. 1 J/cm2 for 16.7 hours. After 10 cycles, thus a total of 167 hours, there were still no signs of adverse effects on the box material. Because the energy of the lamp used is considerably higher than that of a lamp used in a sterile bench, it can be assumed that both boxes can be exposed to UV radiation for at least 300-600 hours in a sterile bench without problems. Because it is well known that polypropylene is at least 3 times more stable against UV radiation than polycarbonate, correspondingly longer UV stability under sterile bench conditions can be presumed for the epTIPS Racks.
Are 1.5 ml Eppendorf Safe-Lock Tubes safe for use in the food industry in Europe?Back to top
"Our 1.5 ml Eppendorf Safe-Lock Tubes are suitable for use in the food industry according to the Food and Consumer Goods Legislation § 5, paragraph 1 no. 1 (food and consumer goods). Please note: The suitability of a consumer item for the intended purpose, the influence of the taste and smell of the filled goods and the conformation to limits (e.g. global migration, specific limits and further analytical requirements) must be checked with the manufacturer or user in individual cases."
What significance does the red dot on the carton for Eppendorf Biopur® products have?Back to top
"It indicates sterilization by radiation. This indicator changes from orange to red when the product has been sterilized with radiation as planned. "
How much serum evaporates from a Eppendorf Safe-Lock Tube at -20 to -70 °C?Back to top
In the case of a well sealed Eppendorf Safe-Lock Tube, evaporation doesn't play a role either at room temperature or at the named temperatures. The material itself has a low level of permeability for steam, but this is insignificant.
Are the non-amber colored Eppendorf Safe-Lock Tubes UV resistant?Back to top
The non-amber colored Eppendorf Safe-Lock Tubes are UV resistant in the sense that DNA samples can be processed in them without danger of DNA chain damage. Samples are generally stored in the refrigerator or in an ice bath, and are therfore usually only very briefly exposed to daylight. We recommend the use of amber colored tubes for particularly light-sensitive samples.
What is the life expectancy of Eppendorf micro test tubes?Back to top
At least 5 years at a storage temperature of +5 to +35 °C.
From what material are your micro test tubes manufactured?Back to top
All of our tubes are manufactured from polypropylene. The polypropylene we use fulfils the specifications of the FDA for the use of plastics for foodstuffs. No metal-based dyes are used.
Do you offer siliconized micro test tubes?Back to top
"Our tube assortment does not include coated or siliconized tubes. In order to keep sample loss caused by the adsorption of sample material on the tube wall to a minimum, we recommend using the Eppendorf Protein LoBind Tubes or Eppendorf DNA LoBind Tubes. These are made of a special type of polypropylene and are manufactured under optimized process conditions. "
Can Eppendorf micro test tubes be sterilized with ethylene oxide?Back to top
"Yes, this is possible. Please note that the micro test tubes have to be taken out of the packaging for sterilization. In addition, please note that some ethylene oxide will remain in the material after sterilization. "
Can your micro test tubes be frozen at -80 °C?Back to top
Yes. Please refer to the operating manual.
Can your micro test tubes be coated with acrylic resin within the framework of a UV polymerization?Back to top
"Yes, the tubes can withstand UV polymerization. However, we cannot give you any instructions for the procedure."
Can contamination of micro test tubes by bacterial endotoxin be inactivated by autoclaving?Back to top
"No. Bacterial endotoxins (lipopolysaccharide, LPS) are very stable and withstand normal autoclaving at 121 °C. To inactivate LPS, the objects have to be baked at 250 °C, which, of course, is not possible with plastics."
Which Eppendorf micro test tubes are free of DNA?Back to top
"All micro test tubes designated with "PCR clean" are free of human DNA, DNase, RNase and PCR inhibitors. Our Safe-Lock micro test tubes are also available in the "Eppendorf Biopur" purity standard. Due to the special manufacturing processes and the individual blister-packaging, Biopur products are certified "DNA free"."
What level of protein binding affinity do your micro test tubes demonstrate?Back to top
Our micro test tubes are used both in the proteomics area and for storage and transport of many different protein solutions. If you work with low protein concentrations, we recommend using the Eppendorf Protein LoBind Tubes. These tubes are made of a special type of polypropylene and are manufactured under optimized process conditions. In comparison to standard polypropylene tubes, these tubes therefore demonstrate a considerably lower affinity to proteins.
How do you define the temperature entry "RT", for example, as a storage condition for your micro test tubes?Back to top
"Definition of RT (room temperature): +5 to +35 °C."
What is the best way to label Eppendorf micro test tubes?Back to top
"The micro test tube should be clean and free from grease and only labeled on the space provided. Water-resistant felt pens from Steadtler S and F, in black, are the most suitable for this. The writing should be left to dry for at least 2 min."
Do your micro test tubes contain heavy metals?Back to top
Lead, cadmium, mercury, etc. are well below the prescribed limits of the DIN norm. In the course of the production process it is impossible to avoid the smallest quantities of metal resulting from machine friction.
Are 1.5 ml Eppendorf Safe-Lock Tubes safe for use in the food industry in USA?Back to top
"The composition of the 1.5 ml Eppendorf Safe-Lock Tube conforms to the specifications of the FDA regulation 21 CFR § 177,1520 "Olefin polymers". Please note: The suitability of a consumer item for the intended purpose, the influence of the taste and smell of the filled goods and the conformation to limits (e.g. global migration, specific limits and further analytical requirements) must be checked with the manufacturer or user in individual cases."
Do Eppendorf Safe-Lock Tubes contain polyethylene glycol (PEG)?Back to top
No. All our test tubes are made from pure polypropylene. The material does not contain PEG.
In which temperature range can the Eppendorf Safe-Lock Tubes be used?Back to top
They can be used from -80 °C to +100 °C. However at temperatures of > 95 °C the tube lids are more likely to pop open. For purposes of autoclaving, the tubes can be heated up to 121 °C.
To what extent are the amber colored Eppendorf Safe-Lock Tubes permeable to fluorescent dyes?Back to top
The amber colored Eppendorf Safe-Lock Tubes protect from light in the spectrum from 700 nm to the UV spectrum of 190 nm. In the spectrum of visible light (350 - 600 nm) the protection is optimal with lessening intensity. At app. 550 nm (excitation wavelength for Cy3) the measured deletion is app. 1, meaning that 90 % of the incident light is blocked by the walls of the tube. At 650 nm (excitation wavelength for Cy5) the measured deletion is app. 0.35, meaning that app. 65 % of the incident light is blocked by the walls of the tube. If that is not sufficient, we recommend wrapping the tube in aluminum foil.
Can Eppendorf micro test tubes be stored in liquid nitrogen?Back to top
"Eppendorf Tubes can be stored up to –200°C in the gaseous phase directly above the liquid nitrogen although we can't guarantee that the tubes stay sealed as it is possible that the lid could open accidentally. WARNING! Do not use Eppendorf tubes for storage in the liquid phase of liquid nitrogen. Such use may cause entrapment of liquefied nitrogen inside the vial and lead to pressure build-up resulting in possible explosion or biohazard release. "
Can the Micro Test Tubes 3810X be stored for 3 months at 90 °C?Back to top
"Yes. The tube material could shrink somewhat, but this should have no negative effect on the tube seal. "
Can the lid of the Standard Micro Test Tube 3810X be pierced as easily as that of the Eppendorf Safe-Lock Tube?Back to top
No. It is more easy to pierce a Eppendorf Safe-Lock Tube because of the thin membrane in the middle of the lid.
From what material is the Micropestle manufactured?Back to top
"The Micropestle is manufactured from polypropylene."
Can the Micro Rack 3821 be stored at -86 °C?Back to top
Yes.
From what material is the Rack 3821 made?Back to top
The Rack 3821 is manufactured from noryl, a polyphenyl ether modified with polystyrene.
What material is the Tube Rack made of?Back to top
It is made of polycarbonate.
Is it possible that nitrogen from the tube material penetrates my sample?Back to top
No. The propylenes which we use are materials of foodstuff quality. It is tested according to the directives for materials which come into contact with foodstuffs of the German Federal Office of Health and has no nitrogen-containing components.
Do the Eppendorf PCR consumables have a special grade of purity?Back to top
Yes. We certify our PC plates and sealing options with "PCR-Clean". This certificate stands for guaranteed freedom from human DNA, DNase, RNase and PCR inhibitors.
Can the deep well mats be autoclaved?Back to top
Yes. They can be autoclaved (1 bar, 20 minutes, 121 °C).
I would like to seal my deep well plate with a heat sealing foil. Can I also puncture the foil with a multi-canal pipette?Back to top
Yes. With the "Pierce-it-Lite" heat sealing foil from Eppendorf this is possible without problems!
Which of the Eppendorf PCR 96 Plates can be sealed with the heat sealer?Back to top
All plates listed in the Eppendorf catalog can be sealed with the heat sealer.
What is the best sealing procedure for storing plates at - 80 °C?Back to top
Heat sealing is the only method which enables a 100% hermetic sealing. We recommend using the heat sealer and the Peel-it-Lite heat sealing foil from Eppendorf, which is suitable for storage temperatures down to – 200 °C. To recover the products, the sealing is simply removed manually.
What is the best way to seal a PCR plate?Back to top
The heat sealer from Eppendorf in combination with heat sealing foils or films offers the best sealing. Another effective method is to use Eppendorf cap strips. For sealing with cap strips, we recommend using the capping aid, an inexpensive tool for producing a very effective seal as cannot be obtained by setting on the caps with the fingers alone.
What are Maxitips for Varipette 4720 made of?Back to top
Maxitips are made of polypropylene.
What are the dimensions of the Maxitips S and G?Back to top
Both tips have the same measurements. The length is 242 mm, the upper base has a diameter of app. 10.5 mm, the diameter of the Maxitip is 10 mm. The diameter of the somewhat elongated tips (25 mm long) is 4.5 mm.
Are the Eppendorf LoBind Tubes autoclavable?Back to top
"Yes, both the Protein LoBind and the DNA LoBind Tube are autoclavable when open (20 min, 121 °C, 1 bar)."
What distinguishes the DNA LoBind Tube from a "Standard" Eppendorf Safe-Lock Tube?Back to top
In comparison to a Eppendorf Safe-Lock Tube, the DNA LoBind Tube shows significantly less DNA bonding affinity. This effect is most apparent with high salt concentrations and when working with short DNA fragments. The DNA LoBind Tube is therefore especially suitable for applications that should be carried out with the minimum loss of nucleic acid material.
Do the Eppendorf LoBind Tubes have the same material durability as all other micro test tubes from Eppendorf?Back to top
"The Eppendorf LoBind Tubes completely satisfy the specifications of the Eppendorf Standard Safe Lock tubes with reference to centrifugation stability (exception: Protein LoBind Tube), lid tightness, pop up safety and chemical resistance. (See Application No. 56: The best material for original Eppis.)"
Are the Eppendorf LoBind Tubes coated?Back to top
Not the Eppendorf LoBind Tubes are neither siliconized nor coated. The low binding affinity results from of a special type of polypropylene and the optimized manucturing process.
Can I use the Protein LoBind Tubes regardless of whether I am working with basic or acidic proteins?Back to top
The Protein LoBind tubes show protein low binding properties for both low and high pH values.
Are Eppendorf Deepwell Plate autoclavable?Back to top
Yes, but the dimensional precision of the plates can be affected. If the use of sterile plates is needed we recommend Eppendorf Deepwell Plate Sterile.
Are Eppendorf Deepwell Plate available with Barcode?Back to top
Yes - Eppendorf Deepwell Plate are optional available with Barcode. Detailed ordering information can be found under www.eppendorf.com/DWP .
Eppendorf Deepwell Plate are withstand high centrifugation forces – what are the limits?Back to top
Eppendorf Deepwell Plate Standard and LoBind Quality can be centrifuged up to 6000 x g. Eppendorf Deepwell Plate Sterile withstand up to 5000 x g. For details refer to the instruction manual.
Are Eppendorf Deepwell Plate LoBind coated or siliconized?Back to top
No - the plates are neither coated nor siliconized.
Does the polypropylene of the plates contain plasticizers?Back to top
No – Eppendorf Deepwell Plate are made of polypropylene which does not contain plasticizers.
What are the precise dimensions of Eppendorf Deepwell Plate?Back to top
Please see for that Plate Dimensions included in the Technical Information (www.eppendorf.com/DWP).
Are Eppendorf Deepwell Plate together with the colored border resistant against organic solvents?Back to top
Eppendorf Deepwell Plate are of polypropylene. All parts of the plate (colorless/colored parts and coding) have the same high resistance against chemicals and are resistant against many organic solvents. For details please see our Application Note no. 56: The best material for original Eppis. In case of doubt please contact Eppendorf Application Support: support@eppendorf.com.
How are the sterile Eppendorf Deepwell Plate packed?Back to top
The plates are packed stacked in a resealable ZipLock bag.
Is it possible to store Eppendorf Deepwell Plate at –196°C in liquid nitrogen?Back to top
No - we recommend storage at –80°C.
Are the ep T.I.P.S. LoRetention autoclavable?Back to top
Yes, they can be autoclaved exactly like the standard tips. Full function will be maintained. The Dualfilter tips cannot be autoclaved.
Under which conditions will the use of ep T.I.P.S. LoRetention be advantageous?Back to top
These tips will grant an advantage especially during pipetting of solutions containing detergents, such as PCR master mixes, enzyme solutions and many buffers. The LoRetention tips are characterized by significantly improved flow performance, thus achieving more economic use of expensive reagents, while valuable sample material is used effectively. Furthermore, reproducibility is improved, since wetting of the tip is dependent on both user and handling (for example: pipetting speed) and can therefore vary considerably.
Are the ep T.I.P.S. LoRetention siliconized or otherwise coated?Back to top
No, the tips are uncoated. The ultra-hydrophobic surface is achievd through an innovative treatment at the molecular level.
Are the ep T.I.P.S. LoRetention resistant to organic solvents?Back to top
The LoRetention tips are made from polypropylene, as are standard tips, and they are resistant to a number of organic solvents. Further information regarding chemical resistance properties of pipette tips can be found in the User Guide no. 23: Chemical Stability of Consumables.
How are the boxes containing ep T.I.P.S. LoRetention distinguishable from those containing other ep T.I.P.S.?Back to top
The lids of the LoRetention racks and reloads have a transparent lid as compared to the blue lid of the standard tips. A reusable LoRetention adhesive sticker is placed on the lid. This sticker also features “LoRetention” writing on the reverse side. This way previously purchased epT.I.P.S. boxes can be labeled. When the box is open, the writing “LoRetention” will thus be easily readable.
Will foam formation, which is observed during pipetting of detergent containing solutions, be reduced when epT.I.PS. LoRetention are used?Back to top
Yes. Foam formation is rarely observed with the use of LoRetention tips. This facilitates the handling of these solutions and increases reproducibility.
Which advantages do Eppendorf Polypropylene Microplates have over plates made from polystyrene?Back to top
Plates made from polypropylene may be used across a broad spectrum of applications, as they are more resistant to temperature variations as well as various chemicals than plates made from polystyrene. Furthermore, they are more robust, allowing them to be centrifuged at higher g-forces. In addition, biomolecules, such as nucleic acids and proteins, bind less strongly to polypropylene.
Are Eppendorf PP Microplates resistant to organic solvents, e.g. DMSO?Back to top
The Eppendorf PP Microplates are made from polypropylene and are thus resistant to DMSO and other organic solvents, unlike plates made from polystyrene. An overview of chemical resistance can be found in Application Note no. 56. In case of doubt please contact Eppendorf Application Support: support@eppendorf.com.
Are the colored rim and labeling resistant to organic solvents?Back to top
All areas of the plate, including the colored rim, are resistant to chemicals. The alpha numeric labeling is equally durable. An overview of chemical resistance can be found in Application Note no. 56. In case of doubt please contact Eppendorf Application Support: support@eppendorf.com.
How may the Eppendorf PP Microplates be sealed?Back to top
Eppendorf offers a variety of sealing options for Microplates. Self-adhesive sealers, films and foils for heat sealing, as well as re-usable sealing mats, are available. Information regarding the compatibility of the different sealing methods is listed in the Application Note no. 208.
Which advantages do the black Eppendorf PP Microplates offer?Back to top
Plates made from black material are especially well suited for fluorescence assays (e.g. PicoGreen®). The black wells prevent “cross talk” between wells and reduce background signals. The black Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in black plates. Due to carefully selected materials, the plates have very low autofluorescence. The superior signal-to-noise ratio contributes to the increased sensitivity of fluorescence assays (see Application Note no. 203).
Which advantages do the white Eppendorf PP Microplates offer?Back to top
Plates made from white material may be used for luminescence assays (chemiluminescence, scintillation proximity assays (SPA) etc.), as well as for fluorescence assays (fluorescence intensity, polarization, etc.). The white wells prevent “cross talk” between the wells while amplifying the individual signals. The white Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in white plates. Due to carefully selected materials, the plates offer maximum signal amplification. Due to their very low autofluorescence, the white Eppendorf Microplates are equally well suited for fluorescence assays. Taken together, these features allow for an improvement in the sensitivity of analyses.
May the Eppendorf PP Microplates be stored at –196°C in liquid nitrogen?Back to top
No, we recommend storage at a minimum of –80°C.
May the Eppendorf PP Microplates be autoclaved?Back to top
Yes; however, the exact specifications of the Microplates could be compromised. The plates are available in the qualities sterile or PCR clean. Hence, autoclaving is necessary only under exceptional circumstances.
Are the Eppendorf PP Microplates in LoBind quality coated or siliconized?Back to top
No, the LoBind quality of the Eppendorf PP Microplates is not derived from siliconization or any other type of coating. The functionality is achieved through a carefully selected material, in combination with a special manufacturing process.
How high may Eppendorf PP Microplates be centrifuged?Back to top
The Eppendorf PP Microplates may be centrifuged up to 6000 x g. Please follow the instructions provided in the manual.
May immunoassays such as ELISA be performed in the Eppendorf PP Microplates?Back to top
Plates made from polypropylene are not suited for the standard variant, where antigen or antibodies are bound to the well wall. For this application, plates made from polystyrene are used, which have a higher binding capacity for biomolecules. However, for antibody-based fluorescence or luminescence assays (e.g. with beads) Eppendorf PP Microplates with black or white wells are suitable, respectively.
Is it possible to perform absorption measurements in the Eppendorf PP Microplates?Back to top
Typically, absorption measurements are not performed in plates made from polypropylene, but in plates made from polystyrene, as this material generally has a very high transparency. Since the Eppendorf PP Microplates show considerably higher transparency compared to other plates made from polypropylene (see Application Note no. 202), the Eppendorf Microplates may be used for absorption measurements in certain cases.
Why are the Eppendorf PP Microplates offered in Sterile and PCR clean, but not in Eppendorf quality?Back to top
The Eppendorf PP Microplates are a very high quality product. In order to underline the quality, all plates are available in the degrees of purity PCR clean or Sterile. This increases the confidence and comfort of the user, who may employ the plates directly for valuable samples in sophisticated applications.
Are Eppendorf PP Microplates also available with barcode?Back to top
Yes, the option of Eppendorf Microplates with barcode is available. Further information can be found on the product page www.eppendorf.com/microplates.
What are Combitips made of?Back to top
The cylinder is made of polypropylene (PP) and the piston is made of polyethylene (PE).
How are the Combitips tested during production?Back to top
Random samples are removed from each batch of Combitips and tested with a calibrated Repeater plus.
What purity grades are available for the Combitips advanced?Back to top
The Combitips advanced are available with the purity grades "guaranteed quality" "pcr clean"(not available in North America) and "biopur"
Which certificates are available for Combitips?Back to top
We are happy to send you a quality certificate for Combitip standard goods. Since Combitips are also available in Eppendorf PCR-clean and Eppendorf Biopur quality, a batch-specific Eppendorf certificate is also available
Are Standard Combitips also sterile?Back to top
No. Although they are packed straight from production, they are not specially sterilized. If you want sterile Combitips, we recommend our Biopur Combitips.
Are Combitips autoclavable?Back to top
No. The Combitip advanced is made of two materials which behave differently during autoclaving. This could lead to leakage which effects the performance of the Combitip
Are the Biopur Combitips tested according to USP and EP?Back to top
Yes they are. This information is also given on the corresponding certificate.
Which adapter is required for the 25 ml Combitip?Back to top
This Combitip advanced requires the red adapter, Ord. No. 0030 089.715, Biopur quality Ord. No. 0030 089.731.
Which adapter is required for the 50 ml Combitip?Back to top
For this Combitip advanced the gray Adapter is needed. Order-Nr. 0030 089.723, Biopur Quality, Order-Nr. 0030 089.740
Can the adapter for the 25 ml and 50 ml Combitip be cleaned using sodium hypochlorite?Back to top
The adapter is made of polypropylene (PP). PP is resistant to sodium hypochlorite for a very long time at room temperature.
Does a Combitip always have to be filled to the total volume?Back to top
No, you can also work with Combitips that are not completely filled. Please note that the 1st dispensing must also be discarded in this case.
Can Combitips be used in food analysis?Back to top
The materials used to produce Combitips (50 ml, 25 ml, 10 ml, 5 ml, 2.5 ml, 1 ml and 0.5 ml) are compliant with the requirements on materials used for articles or components of articles intended to come into contact with food as described in:
France: Repression des Fraudes (1997), No 1227, et Supplément N° 1
EU: Commission Directive 90/128/EEC, 92/39/EEC, 93/9/EEC, 95/3/EC and 96/11/EC, Section A
USA: FDA, CFR, Title 21 (1998), 177.1520(a)(3)(i)(c)(1), (b) and (3)3.1a Olefin polymers.
Detection limits for monomers and additives used are not included in the directives mentioned. As Eppendorf does not use any additives and/or monomers in the production of Combitips, there should not be any problems.
Are Combitips resistant to nonane and dodecane?Back to top
These liquids have not been tested separately. The test result for petrol, which contains both these components, shows that use at room temperature is possible for 10 min to several hours. It should therefore be possible to pipette these substances.
Are Combitips resistant to acetonitrile?Back to top
Yes, however bleaching of the scale could occur in the long term.
Are Combitips resistant to acids?Back to top
Combitips advanced are resistant to most moderate inorganic acids. For example, 10% nitric acid, 60% sulphuric acid, 40% hydrofluoric acid and 35% hydrochloric acid can be taken up without damaging the Combitip and can be dispensed over a period of several weeks. We are not, however, able to give any information about dispensing accuracy or functionality of liquid dispensing. Also, we are not able to make any generally applicable statements for organic acids since the resistance varies considerably, depending on the acid. For example, the Combitip is resistant to 50% acetic acid at room temperature, but not to butyric acid.
Are Combitips resistant to trifluoroacetic acid?Back to top
This substance was tested separately. The test result for trifluoroacetic acid with a concentration below of 50% shows that Combitips can be used. Therefore higher concentrated trifluoroacetic acid (> 50%) the resistant is dependent on the exposure time.
Are Combitips resistant to PEG 400 (polyethylene glycol)?Back to top
This substance was not tested separately. The test result for ethylene glycol on PE and PP shows that Combitips can be used. Pipetting of PEG should therefore also be possible.
Are the Combitips resistant against petroleum spirit N28?Back to top
The primary components of petroleum spirit are pentane, hexane and octane. Because the secondary components are unknown, we cannot guarantee that the Combitips are suitable for your application. However, we would be pleased to send you a sample of the Combitips to try out.
Can I aspirate liquid from a 1 liter bottle with a 50 ml Combitip without filling the liquid into a beaker first?Back to top
Yes. For this we recommend the "Combilong". It consists of a long suction tube that can be cut to the corresponding bottle depth. A reservoir is filled through this tube from which liquid can be drawn easily with the Combitip.
Ordering-Nr. 0030 059.506
Can the Combilong be autoclaved?Back to top
Yes. At 121 degrees Celsius.
What are Combitip Racks made of?Back to top
The Combitip rack is made of polypropylene (PP) and is autoclavable.
Are Combitip Racks autoclavable?Back to top
Yes, up to 50 times.
When must the Combitips advanced adapter for 25 ml and 50 ml have to be changed?Back to top
The Combitips adapter are wear parts. They should be changed regularly. Worn-out adapters might get caught when ejecting the combitip.
Are Combitips advanced free from slip agents?Back to top
Yes, Combitips advanced are produced without using slip agents.There is a batch independant certificat available.
Is there a certificat available regarding trace metalls?Back to top
Yes, there is a lot unspecific certificat available.
Is there a certificat available regarding slip agents (including oleamide, erucamide, stearamide),biocides (including DiHEMDA), plasticizers (phthalates),BSE/TSE free?Back to top
Yes, there is a lot unspecific certificat available.
What types of applications can be carried out with Eppendorf Tubes® 5.0 mL?Back to top
Basically, this tube type can be used for the same applications as the micro centrifugation tubes available in 1.5 and 2 mL formats. They close a gap between this small tube format and conical 15 mL tubes for sample volumes up to 5 mL. The tubes can be used in the field of cell culture. For this purpose, they are available in the purity grades “Sterile” (= sterile and pyrogen-free) or Biopur. They are suitable for lysis of cells and tissues and purification of nucleic acids and proteins. For this, the PCR clean purity grade and LoBind variants are provided. They are also ideally suited for the preparation of large volumes of master mixes, buffers or dilution series as well as for space-saving storage of samples.
Is it necessary to buy new equipment to be able to use the new tube?Back to top
The bottom shape of the Eppendorf Tubes® 5.0 mL is similar to that of conical 15 mL tubes which means that they are compatible with a wide range of standard laboratory equipment. In addition, an extensive range of accessories is available, enabling them to be used in many devices. Both racks and storage boxes are available for lab bench work and storage. Incubation and mixing operations can be performed using suitable blocks on Eppendorf thermomixers and compatibility with Eppendorf centrifuges is ensured by special rotors and a variety of adapters. With a suitable reservoir rack module, the tubes can also be integrated into the epMotion® automated liquid handling workstation. A detailed description of the accessories is provided in Application Note No. 265.
What are the advantages of using Eppendorf Tubes® 5.0 mL compared with conical 15 mL screw-cap tubes?Back to top
The compact, space-saving design greatly facilitates the processing of samples with volumes in the range of 2-5 mL. Eliminating the risk of dipping the tip cone into the tube avoids contamination. The snap-cap lid allows one-hand operation and the tubes can withstand forces of up to 25,000 x g for time-saving and effective centrifugation. A further advantage is that no components are released from the material of the Eppendorf Tubes which could affect photometric detection methods (Application Note 264). A lot of information on using the tubes can be found in Application Note 265.
Is it possible to store samples in 5.0 mL Eppendorf Tubes® in the freezer?Back to top
Yes, the tubes can be stored down to -86 °C.
How resistant are the Eppendorf Tubes® 5.0 mL to chemicals?Back to top
The tubes are made of high quality highly transparent polypropylene and therefore resistant to a variety of commonly used chemical substances in the laboratory. An overview is provided in Application Note No. 56.
Are the Eppendorf Tubes® 5.0 mL available with the same purities and LoBind variants as the small micro centrifugation tubes from Eppendorf?Back to top
The Eppendorf Tubes® 5.0 mL are available in the purity grades Eppendorf Quality, PCR clean and Biopur® and also in the purity grade Sterile. In addition to irradiation for sterilization, this also includes certified testing for the absence of pyrogens. The tubes are also available as DNA LoBind and Protein LoBind variants.
Can the universal adapter for conical 15 mL screw-cap tubes be used in each rotor/adapter that is designed for this tube type?Back to top
It can be used in the respective Eppendorf rotors/adapters, but we assume no liability for use in rotors/adapters from other manufacturers.
What is the Tube Clip used for?Back to top
The Eppendorf Tubes® 5.0 mL offer secure closure at incubation temperatures up to 80 °C. For higher temperatures, the Tube Clip should be used to secure the lid.
Up to which g-force can the Eppendorf Tubes® 5.0 mL be centrifuged?Back to top
All tube types are resistant up to 25,000 x g when used in combination with a suitable rotor or adapter. (Test conditions: 45° fixed-angle rotor at 40 °C, with aqueous salt solution (density 1.0 g/mL) and 90 mins). The mechanical loading capacity of the tubes is reduced by the use of organic solvents.
Which cleaning and disinfection agents can be recommended for cleaning and disinfection of ThermoMixer C, FP, F1.5 and ThermoStat C?Back to top
Do not use corrosive cleaning agents, aggressive solvents or abrasive polishes. Do not use any aggressive chemicals on the device or its accessories, such as strong and weak bases, strong acids, acetone, formaldehyde, halogenated hydrocarbons or phenol. If the device becomes contaminated with aggressive chemicals, clean it immediately with a mild cleaning agent. Use Lint-free cloth, mild, soap-based lab cleaner and dist. Water. Please refer to your operating manual chapter Maintenance. If you have further questions you might contact our Application Support Team under support@eppendorf.com
Is the housing of ThermoMixer C, FP, F1.5 and ThermoStat C stable against UV irradiation?Back to top
A disinfection by UV irradiation cannot be recommended, because discoloration of the housing materials can occur.
Which DNA and RNAse deconatmination agents can be recommended for decontamination of the devices and the SmartBlocks?Back to top
We tested DNA away and RNase away and we can recommend to use both agents.
Which decontamination agent do you recommend to remove radioactivity from the devices and SmartBlocks?Back to top
We recommend to use Count off.
Are start and end position of the SmartBlocks the same?Back to top
No. Start and end position are not identical.
How can the temperature accuracy be verified?Back to top
The best possible temperature accuracy can be guaranteed by individually adjusting a SmartBlock. Optimized temperature control for each block type keeps temperature overshoots and deviations caused by external interferences to a minimum. To guarantee optimal performance over the entire period of use, Eppendorf offers a preventive maintenance program and certification services: your Eppendorf ThermoMixers, ThermoStat C as well as the SmartBlocks are regularly cleaned, checked and adjusted. Additional information is available at: www.eppendorf.com/epservices. Customers can also verify the temperature accuracy by themself. Eppendorf provides for that purpose the Temperature Verification SystemSystem – Single Well for Eppendorf Mastercyclers (0055 000.298) in combination with the 384 well Sensor (5331 233.007).
Can cell culture plates be used in the SmartBlock plates?Back to top
Also cell cultuture plates can be used in the SmartBlock plates in so far the dimensions of the plate comply to the SBS standard.
Can ThermoMixer C, F1.5, FP & ThermoStat C be operated with remote controle?Back to top
No. The devices can not be contolled remote via a PC.
Can ThermoMixer C, F1.5, FP & ThermoStat C be used in a cold room?Back to top
Yes, one can use the devices in a cold room but some safety hints should be attended: Condensate can form in the device after it has been moved from a cool environment to a warmer environment. Wait at least 3 hours after positioning the device. Plug the device only after this time has elapsed. In case the device is positioned continually in a cold room and was switched off for a longer time – over night for instance - switch it on for at least 1 h before starting a mixing process.
Is it possible to put the SmartBlocks for a rapid cool down into a -20°C freezer?Back to top
The SmartBlocks can be used at ambient temperatures of 5°C – 40°C and can be cooled appropriate. Cooling down of SmartBlocks in a freezer should not be done.
ThermoMixer C; ThermoStat C: Is it possible to run more than one program in automatic succession?Back to top
No. Each program has to be chosen and run separately. But the 4 possible steps within a program run in automatic succession.
Which SmartBlocks and vessels can be used with ThermoMixer F1.5 and FP?Back to top
ThermoMixer F1.5 and FP possess a fix installed SmartBlock. ThermoMixer F1.5 has a fixed installed SmartBlock 1.5 mL and ThermoMixer FP has a fixed SmartBlock plates. Therefore with ThermoMixer F1.5 one can only use 1.5 mL tubes and with ThermoMixer FP only MTP’s, DWP’s or skirted plates complying the SBS standard. For using PCR plates our SmartBlocks PCR 96 and PCR 384 in combination with ThermoMixer C is the proper choice.
The ThermoMixer / ThermoStat shows the error message “ThermoTop not recognized”.Back to top
Please check if a compatible SmartBlock has been attached and the ThermoTop has been vertically placed from above. The centering pins behind the heating/cooling plate have to fit perfectly into the recesses of the ThermoTop. Place the ThermoTop once again upon the device. In case the device shows still the error message, please contact the technical service.
Can PCR plates be used in SmartBlock plates respectively ThermoMixer FP?Back to top
SmartBlock plates and ThermoMixer FP (with the fixed SmartBlock plates) are dedicated for using MTP’s, DWP’s and also other skirted plates which meet the SBS standard. For optimal usage of PCR plates the SmartBlock PCR 96 and PCR 384 in combination with ThermoMixer C or ThermoStat C is the proper choice. These SmartBlocks possess conical bores which surround the conical wells of PCR plates properly for a perfect temperature transition.
Can the ThermoStat C be used in a cold room?Back to top
Yes, one can use the devices in a cold room but some safety hints should be attended: Condensate can form in the device after it has been moved from a cool environment to a warmer environment. Wait at least 3 hours after positioning the device. Plug the device only after this time has elapsed.
The ep T.I.P.S. Dualfilter have the highest efficiency in the market. Has this changed with the ep T.I.P.S. SealMax?Back to top
No. The Dualfilter of ep Dualfilter T.I.P.S. SealMax is built upon the existing Dualfilter technology. Thus there is no decrease in filter efficiency.
If I did a mistake the sample touched the filter and the filter blocks. Do I have to throw away the sample together with the tip?Back to top
No. You can recover nearly 100% of your sample! Simply press down the operating button of you pipette and dispense the liquid. The recovery rate has been shown in Application Note 273.
Has the position of the filter changed if compared to ep Dualfilter T.I.P.S. ?Back to top
No. The filter of ep Dualfilter T.I.P.S. SealMax is in the same position as the ep Dualfilter T.I.P.S.
Is there any difference of aerosol penetration rate in comparison to ep Dualfilter T.I.P.S?Back to top
No. The Dualfilter of ep Dualfilter T.I.P.S. SealMax is the same HEPA class H12 according to EN 1822 (99.5 % of aerosols are retained)
Does the self-sealing mechanism of the filter work with all kinds of liquids?Back to top
No. The self-sealing mechanism works best with aquaeous solutions, most acids and bases. We recommend our ep Dualfilter T.I.P.S. LoRetention, when working with detergent containing solutions.
May I use the ep Dualfilter T.I.P.S. SealMax for several pipetting processes, also if the filter came into contact with the liquid?Back to top
No. Filter tips in general are meant to be used only once as they are used to avoid contaminations. Therefore we recommend to always use a new filter tip when starting the next pipetting process.
Is there a time limit for the recovery of sample liquid when accidentally over-pipetting happened?Back to top
Generally a pipetting process takes some seconds of time between absorbing the sample and dispensing it. The Dualfilter of the ep Dualfilter T.I.P.S. SealMax gives you at least 60 seconds to recover your sample safely.
 
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